|
KMID : 1134820080370080979
|
|
Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 8 p.979 ~ p.984
|
|
|
Isolation and Identification of Antioxidant Substances from the Stems of Butterbur (Petasites japonicus)
|
|
Kim Min-Young
Lee Jung-Hyun
Hwang Yun-Yi
Song Kyung-Sik
Jun Mi-Ra
|
|
|
Abstract
|
|
|
The stems of Petasites japonicus were extracted with ethanol and then partitioned with hexane, chloroform,
ethyl acetate, n-butanol and water, successively. The antioxidant potency of five crude fractions were
determined using (1) 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, (2) thiobarbituric acid
reactive substances (TBARS) assay in the linoleic acid model system, and (3) lipoxygenase inhibition assay.
Among the crude fractions, the ethyl acetate fraction exhibited the most potent antioxidant effect. By
activity-guided fractionation, compound PJ-4 was isolated from the ethyl acetate fraction through the repeated
silica gel open column chromatography. The chemical structure of the isolated compound was determined as
kaempferol by 1H- and 13C-NMR analysis and its antioxidative capacity was further investigated. DPPH radical
scavenging activity of the compound was 65.76% at the concentration of 100 μg/mL. The inhibitory activity
of the compound against lipid peroxidation and lipoxygenase exhibited 43.47% and 58.60%, respectively at
the concentration of 100 μg/mL. The result suggests that the compound may serve as a useful natural
antioxidant and furthermore indicates the possibility of developing the stems of Petasites japonicus as a natural
antioxidant source.
|
|
|
KEYWORD
|
|
|
Petasites japonicus, kaempferol, antioxidant, TBARS, lipoxygenase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|